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Nagarjun

     

    EDUCATION

    • Aug 2010-Dec 2015 : PhD in Bioorganic Chemistry; Thesis title. “Design, synthesis and applications of small molecular probes in studying canonical and non-canonical DNA structures”. Supervisor: Prof. T. Govindaraju, New Chemistry Unit, JNCASR, Bangalore, Karnataka, India
    • Aug 2008-Jun 2010 : M. Sc in Chemistry with First class, University of Hyderabad, Hyderabad, Telangana, India.
    • Aug 2005-Jun 2008 : B.Sc (MPC) with Distinction, Andhra Pradesh Residential Degree College (APRDC), Nagarjuna Sagar, Acharya Nagarjuna University, Guntur, Andhra Pradesh, India.

    Academic Achievements

    • Secured All India 26th rank in National Eligibility Test (NET) and called for Syama Prasad Mukherjee (SPM) interview for Junior Research Fellowship (JRF) in June-2010 conducted jointly by the Council of Scientific and Industrial Research (CSIR) and University Grants Commission (UGC).
    • Qualified for GATE (Graduate Aptitude Test in Engineering) conducted by Indian Institute of Technology (IIT)-Guwahati in 2010 ( All India 165th Rank, percentile of 98.00 %).
    • Secured All India 10th rank in the entrance exam followed by personal interview, for admission into Master of Science in Chemistry in 2008 conducted by University of Hyderabad (UoH), India.
    • Won the Gold Medal in 2008 for the highest marks achieved in Bachelor of Science from the Andhra Pradesh Residential Degree College (A.P.R.D.C), India.

    Research Interests

    • Design and synthesis of Red and NIR fluorescence probes for nucleic acids (dsDNA, RNA and G-quadruplex structures).
    • Stimuli responsive molecular probes for sensing and in vivo imaging applications in cells.
    • Small molecules conjugated DNA-nanodevices for metal ions sensing applications in cells.

    Experimental skills

    • Multi-step synthesis, solid-phase peptide synthesis, purification & spectral characterizations of organic molecules, cationic lipids, small peptides, Red and NIR-fluorescence molecules in milligram to gram scales.
    • Hands-on experience in high resolution NMR (400 MHz), IR, UV-VIS spectrophotometer, LC-MS, GC-MS, HPLC (Normal Phase, RP-C4 and C18), automated flash chromatography.
    • Multiple characterization techniques to confirm the small molecule-DNA interactions using various tools including UV-Vis absorption spectroscopy, fluorescence spectroscopy, circular dichroism spectroscopy, Atomic Force microscopy (AFM), gel-electrophoresis (Agarose and PAGE) of DNA.
    • Culture and maintenance of various mammalian cell lines such as CHO, MCF-7, HeLa, U251, HacaT, HeLaDox, A549, MDA-MB 231 etc; MTT-based cytotoxicity assays etc.

    Scientific publications

      1. Narayanaswamy, N.; Nair, R. R.; Suseela, Y, V., Saini, D. K.; Govindaraju, T. Molecular Beacon-based DNA Switch for Reversible pH sensing in Vesicles and Live Cells. Chem. Commun., 2016 (In press)

      2. Narayanaswamy, N.; Narra, S.; Nair, R. R.; Saini, D. K.; Kondaiah, P.; Govindaraju, T. Stimuli-responsive colorimetric and NIR fluorescence combination probe for selective reporting of cellular hydrogen peroxide. Chem. Sci., 2016, 7, 2832-2841.

      3. Rajasekhar, K.; Naryanaswamy, N.; Murugan, N. A.; Kuang, G.; Ågren, H.; Govindaraju, T. A highly affinity red fluorescent and colorimetric probe for Amyloid b aggregates. Sci. Rep., 2016, 6, 23668

      4. Narayanaswamy, N.; Das, S.; Samanta, P. K.; Banu, K.; Sharma, G. P.; Mondal, N.; Dhar, S. K.; Pati, S. K.; Govindaraju, T. Sequence-specific recognition of DNA minor groove by an NIR-fluorescence switch-on probe and its potential applications. Nucleic Acids Res., 2015, 43, 8651-8663.

      5. Narayanaswamy, N.; Suresh, G.; Priyakumar, U. D.; Govindaraju. T. Double zipper helical assembly of deoxyoligonucleotides: mutual templating and chiral imprinting to form hybrid DNA ensembles. Chem. Commun. 2015, 51, 5493-5496.

      6. Narayanaswamy, N.; Unnikrishnan, M.; Gupta, M.; Govindaraju, T. Fluorescence reporting of G-quadruplex structures and modulating their DNAzyme activity using polyethylenimine-pyrene conjugate. Bioorg. Med. Chem. Lett. 2015, 25, 2395-2400.

      7. Narayanaswamy, N.; Kumar, M.; Das, S.; Sharma, R.; Samanta, P. K.; Pati, S. K.; Dhar, S. K.; Kundu, T. K.; Govindaraju. T. A thiazole coumarin (TC) turn-on fluorescence probe for AT-base pair detection and multipurpose applications in different biological systems. Sci. Rep. 2014, 4, 6476.

      8. Narayanaswamy, N.; Avinash, M. B.; Govindaraju. T. Exploring hydrogen bonding and weak aromatic interactions induced assembly of adenine and thymine functionalised naphthalenediimides. New J. Chem. 2013, 37, 1302-1306.

    Poster Presentations

    • International Symposium on Bioorganic Chemistry (ISBOC-10), IISER-Pune, January 11-15, 2015. Poster Title: “ Double zipper helical assembly of deoxyoligonucleotides: mutual templating and chiral imprinting to form hybrid DNA ensembles”.
    • Humboldt Kolleg, IISC-Bengalure, September 4th-6th, 2014. Poster Title: “A Thiazole coumarin (TC) turn-on fluorescence probe for AT-base pair detection and multipurpose applications in different biological systems” ( Selected for oral presentation).
    • Indo-German Conference in Bioinspired Chemistry (IGCBIC-2014), IISC-Bengaluru, September 10th-12th, 2014.Poster Title: “ A Thiazole Coumarin (TC) turn-on fluorescence probe for AT-base pair detection and multipurpose applications in different biological systems”.
    • International conference on chemical biology (ICCB-2014), IICT-Hyderabad, February 6th-8th, 2014. Poster Title: “Base pair specific DNA probe for nuclear staining and cell cycle analysis”.
    • Winter school-2014 on Frontiers in Materials Science, Jointly conducted by University Cambridge and JNCASR (ICMS)-Bengaluru, December 1st-5th, 2014. Poster Title: “ Double zipper helical assembly of deoxyoligonucleotides: mutual templating and chiral imprinting to form hybrid DNA ensembles”.
    • Ninth JNC research conference on Chemistry of Materials, Thiruvananthapuram-Kerala, October 14th-16th, 2013. Poster title: “Highly selective red fluorescent probe for base pair specific DNA recognition and nuclear staining” ( Selected for oral presentation).

Post-doctoral Scholar, Department of Chemistry, University of Chicago,
GCIS, E347A, 929, East 57th St, Chicago, IL 60637
Email: nagarjunn6[at]uchicago.edu
Phone: +1 7737024364


 

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